Alternative Description: Cytokeratin (keratin) type I ( AE1)
Catalogue Number: IQ288
Also known as: CK 1; CK 9; Cytokeratin9; EPPK; Hair alpha protein; K9; Keratin 1; Keratin 9; Krt9; Keratin9;
Quantity: 0.2ml
Price: €328.00Price excludes VAT and Delivery.
Clone: AE1;
Host/Isotype: Mouse IgG1;
Immunogen: Human epidermal keratins
Myeloma/fusion partners: P3 x 63 Ag8 myeloma cells;
Specificity: Most human acidic (type I) keratins K10, K14, K15, K16, K19
Purification: Protein G;
Format: Purified antibody containing PBS + 0.1% sodium azide;
Applications: EM; IB; IHC;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however as guideline try at 1:1000-1:3000 for western blot
Relevance: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. The basic keratins have molecular weights of 65 - 67, 64, 59, 58, 56 and 52kDa. Members of acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with cell type, differentiation status and environment. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
References: (click to view full details)
Classification, expression, and possible mechanisms of evolution of mammalian epithelial keratins- Sun TT, Eichner R, Cooper D, Schermer A, Nelson WG, and Weiss RA ( In The Cancer Cell: The Transformed Phenotype,1984)
Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies.- Tseng SC ( Cell,Sep 1992)
Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells.- Schermer A ( J Cell Biol,Jul 1986)
Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies- Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT ( Journal of Cell Biology,1982)
Monoclonal antibody analysis of bovine epithelial keratins. Specific pairs as defined by coexpression- Cooper D, Sun TT. ( Journal of Biological Chemistry,1986)
Monoclonal antibody analysis of keratin expression in epidermal diseases: a 48- and 56-kdalton keratin as molecular markers for hyperproliferative keratinocytes- Weiss RA, Eichner R, Sun TT ( Journal of Cell Biology,1984)
Research areas: Cell Biology; Cytokeratins; Cytoskeleton; Cytoskeleton / ECM; Intermediate Filaments;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
