Alternative Description: Emerin
Catalogue Number: IQ225
Also known as: EDMD; EMD; Emery Dreifuss muscular dystrophy; STA;
Quantity: 20μl
Price: $365.00Price excludes VAT and Delivery.
Clone: N/A;
Host/Isotype: Rabbit;
Immunogen: Recombinant human Emerin (residues 1-70)
Specificity: Human Emerin
Purification: Affinity purified against residues 1-70 of recombinant human emerin;
Format: Purified antiserum in (PBS + 10% gelatin) + 0.1% sodium azide;
Applications: IB; IF; IHC; IP;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration. The following are suggestions only; For immunoblotting use at 1:3000 - works on rodent and primate tissues and cell lines. For immunoprecipitation from primate and rodent cell lines at 1:100. For immunofluorescence on rodent (but not hamster - CHO) and primate cell lines 1:500. For immunohistochemistry on primate sections 1:100-1:500. For immunohistochemistry on rodent cell lines is poor for mouse/rat 1:50, and doesn't work for hamster.
Relevance: Emerin is a serine rich nuclear membrane protein and a member of the nuclear lamina associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the Emerin gene.
References: (click to view full details)
Aberrant intracellular targeting and cell cycle-dependent phosphorylation of emerin contribute to the Emery-Dreifuss muscular dystrophy phenotype.- Ellis JA ( J Cell Sci,Mar 1998)
Pleomorphism of the Nuclear Envelope in breast cancer: a new approach to an old problem- Bussolati G et al ( J Cell Mol Med,2007 Dec 5)
Research areas: Cell Biology; Class V Lamins; Cytoskeleton; Cytoskeleton / ECM; Intermediate Filaments; Lamins;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
