Alternative Description: Lamin A/C
Catalogue Number: IQ332
Also known as: 70 kDa Lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; HGPS; IDC;
Quantity: 1ml
Price: $295.00Price excludes VAT and Delivery.
Clone: JOL2;
Host/Isotype: Mouse IgG1;
Immunogen: Recombinant human Lamin A
Myeloma/fusion partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP20/Ag4 myeloma cell line;
Specificity: The antibody reacts with both recombinant and native Lamin A and C in humans. It has been shown to bind to an epitope between amino acids 464-572
Purification: Unpurified;
Format: Tissue culture supernatant containing 0.2M Tris/HCl pH7.4 and 510% foetal calf serum;
Applications: FC; IHC; IP; WB;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however as a guideline for western blot up to 1/200, and for immunoprecipitation use neat
Relevance: Nuclear Lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type Lamins and B-type Lamins. The A-type Lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. Lamin A, Lamin C and Lamin Adel 10, while the B-type Lamins include two proteins arising from two distinct genes, i.e. Lamin B1 and Lamin B2. Recent evidence has revealed that mutations in A-type Lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type Lamins coincides with cell differentiation and as A-type Lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type Lamins.
References: (click to view full details)
Both emerin and lamin C depend on lamin A for localization at the nuclear envelope.- Vaughan A ( J Cell Sci,Jul 2001)
Research areas: Cell Biology; Class V Lamins; Cytoskeleton; Cytoskeleton / ECM; Intermediate Filaments; Lamins; Nuclear Envelope; Nucleus; Subcellular Markers; Tags & Cell Markers;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
