Alternative Description: HEF-1 (Cas-L)
Catalogue Number: IQ297
Also known as: CASL; dJ49G10.2; dJ761I2.1; HEF 1; NEDD9; NEDD9 protein; NY REN 12 antigen; p105; Protein NEDD9;
Quantity: 0.1ml
Price: €328.00Price excludes VAT and Delivery.
Clone: 2G9;
Host/Isotype: Mouse IgG1 kappa;
Immunogen: GST-HEF-1(82-398aa.)
Myeloma/fusion partners: SP2/0;
Specificity: Human, mouse, rat HEF-1. Does not recognize p130Cas. Have not tested on Sin1. This mAb mostly detects HEF1 localized at the focal adhesion sites
Purification: Protein G;
Format: Purified antibody containing PBS + 0.1% sodium azide;
Applications: IF; IHC (Human); IP; WB;
View Applications KeyDilutions: Optimal antibody dilution should be determined by titration, however as a guideline try at 1:2000-1:6000 for western blot, 1:1000 for immunoprecipitation & 1:500 for immunofluorescence
Relevance: HEF1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases; two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.
References: (click to view full details)
Nedd9/Hef1/Cas-L mediates the effects of environmental pollutants- L-C Bui et al ( Oncogene ,2009)
Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics.- O'Neill GM ( Molecular and Cellular Biology,Aug 2001)
The Cas family docking protein, HEF1, promotes the formation of neurite-like membrane extensions - Sharmilla D. Bargon et al ( Biochimica et Biophysica Acta (BBA) - Molecular Cell Research,15 December 2005)
The Hematopoietic Isoform of Cas-Hef1-Associated Signal Transducer Regulates Chemokine-Induced Inside-Out Signaling and T Cell Trafficking - Adam G. Regelmann et al ( Immunity,December 2006)
Research areas: Apoptosis; Cell Biology; FAK Family; Intracellular; Transcription;
Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer
Health and safety: Products that contain sodium azide (a poisonous and hazardous substance) should be handled only by trained personnel
Protocols: Immunofluorescence protocol - Formaldehyde fixation; Immunofluorescence protocol - Methanol/acetone fixation; Western Blotting Protocol;
